COLUMNS
SAMPLE PREP PERSPECTIVES
"Just-Enough" Sample Preparation: A Proven Trend in Sample Analysis
Ronald E. Majors
By achieving selectivity in other portions of the analytical cycle, we can lower the burden on sample preparation.

LC TROUBLESHOOTING
Avoiding Refractive Index Detector Problems
John W. Dolan
A description of how refractive index detectors work and some good practices to get the most out of this powerful detector

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BIOTECHNOLOGY TODAY
The Use of Light-Scattering Detection with SEC and HPLC for Protein and Antibody Studies, Part II: Examples and Comparison to Mass Spectrometry
Simion Kreimer, John Champagne, Anurag S. Rathore, and Ira S. Krull
Detailed examples of light-scattering techniques are presented as well as how their performance compares to MS and other methods.

THE ESSENTIALS
Ion-Trap and Time-of-Flight Mass Analyzers
A review of the principles of these analyzers and their relative advantages and disadvantages over other mass analyzer types

ARTICLES
Maximizing Robustness and Throughput in Liquid Chromatography by Using Pressure-Controlled Operation
Matthias Verstraeten, Ken Broeckhoven, Klaus Witt, Konstantin Choikhet, Monika Dittmann, and Gert Desmet
Analysis time can be reduced 10–30% by switching from constant flow-rate mode to a constant-pressure gradient-elution mode.

Elevated Temperatures in Liquid Chromatography, Part III: A Closer Look at the van 't Hoff Equation
David S. Jensen, Thorsten Teutenberg, Jody Clark, and Matthew R. Linford
An exploration of the usefulness and relevance of the van 't Hoff equation using various examples from the literature

Phyllis Brown: A Story of Serendipity, Perseverance, Vision, and Chromatography
In the 1960s, Phyllis Brown dared to do what few chromatographers did: to apply high performance liquid chromatography — then still in its infancy — to real samples, particularly complicated ones like blood. In a new interview, Brown talks about what it was like to be a chromatography pioneer, and how she figured out what she had to do to get recognition in a male-dominated field.

Breaking Ground in Protein Column Efficiency with Slip Flow Technology
The HPLC 2012 conference was abuzz with talk of "slip flow" following the talk given by Mary Wirth of Purdue University about her groundbreaking work involving protein column efficiency. In this interview, Wirth explains how “slip flow” works and what this discovery can contribute to the application of LC-MS to disease research.

Applying LC-MS to Disease and Wellness Diagnosis
LCGC recently spoke with Fred E. Regnier, the John H. Law distinguished professor in the Department of Chemistry at Purdue University (West Lafayette, Indiana) and a member ofLCGC's editorial advisory board. Regnier is known as an innovator and has received 45 patents related to the separation and detection of proteins and peptides. He has also done extensive work in proteomics and is the 2013 recipient of the ACS Award for Separations Science and Technology.

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