The LCGC Blog

HPLC Diagnostic Skills II – Tailing Peaks

Tailing peaks create issues with resolution, quantitation (integration), and reproducibility. Peak shape is often the controlling factor when optimizing complex separations, especially when components are present in very different concentrations. Learn more

Maximize LC Separation Performance
Incorporating core-shell technology, Shimadzu’s Velox HPLC columns enable users to achieve increased separations and faster analysis times on any LC platform, while providing excellent lifetime for the most challenging sample matrices. Utilize Velox columns and achieve improved separation and detection, outstanding analysis and data integrity, reduced analysis costs, higher productivity, and increased sample throughput. Learn more

Exclusive Interview from HPLC
Latest Advancements and Benefits of micro Pillar Array Columns. Watch Now
Sponsored by PharmaFluidics

Featured Articles

Minimizing Fluctuating Peptide Retention in 2D-LC: How to Address a Moving Target

In this installment, tips, tricks, and suggestions are provided for best practices in reversed-phase separations, in both 1D and 2D LC, with gradient elution for minimum variations in retention time. Learn more

Micro Pillar Array Column Technology Resource Center
An online resource with useful tools and practical information for separation scientists who want to find out more about how micro-pillar array columns can benefit the laboratory scientist in practice. The site provides useful technical notes, video interviews, and executive summaries of cutting-edge applications from leading practitioners in the field of proteomics and biopharmaceutical applications. Learn more

Ultraviolet Detectors: Perspectives, Principles, and Practices

The authors provide a technical overview of the design and operating principles of variable wavelength and photodiode array detectors, and include historical perspectives and common practices in operation and maintenance. Learn more

Tools and Information for Assured Data Integrity and Regulatory Compliance
FDA and other regulatory bodies have continued to focus on Data Integrity, which has globally elevated the profile of laboratory compliance. Laboratories in many regulated industries must find a way of balancing continued operation of the lab with performing essential gap analysis and lab improvement projects to satisfy compliance and data integrity requirements. Access a series of resources to help you understand the latest regulatory trends and approaches for assured data integrity and laboratory compliance. Learn more

Recent Advances in Hyphenated Chromatography and Mass Spectrometry Techniques and Their Impact on Late-Stage Pharmaceutical Development

In late-stage pharmaceutical development a new generation of high-resolution mass spectrometers and ion mobility mass spectrometers operate as orthogonal separation techniques and have greatly increased the ability to resolve impurities and increase the level of analytical information gained from a single analysis. Learn more