LCGC North America e-Separation Solutions
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LCGC North America | e-Separation Solutions
May 19, 2016
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The LCGC Blog

Large-Molecule Analysis for Small-Molecule People
I speak with many “small-molecule” folks who are moving into biomolecule analysis and are dismayed at the heterogeneity of proteins and the variety of techniques required to characterize them. My work in recent years has taught me to take a high-level view on the subject and cut through the more esoteric and specialized applications to focus on what is more routine in biomolecule characterization. For those of you faced with moving into this type of analysis or just for interest, here is my guide to characterizing protein biologics.
Automated Solid Phase Extraction System for High Throughput PFC analysis
The TurboTrace® PFC (Perfluoralkylated substances) SPE system is designed to streamline your laboratory’s workflow and increase productivity by automating the manual steps in your sample preparation process. It is designed for high throughput and tough sample matrices. It isthe first automated solid phase extraction system made specifically for PFC extraction and concentration. Effective at reducing background contamination, extraction and concentration of aqueous samples takes less than two hours. Learn more
LCGC TV

Buying a Mass Spectrometer: Questions of Resolution and Ion Mobility?
If you can afford more resolution for your mass spectrometer it’s always a good idea to have some in reserve. Barbara Larsen from DuPont Central Research and Development discusses how you should decide how much resolution you need. She also offers advice on the benefits of ion mobility for mass spectrometry.
Glycan Permethylation µ-Reactor™ Columns
New! Glycan Permethylation µ-Reactor™ Empty Columns with caps (SUM 0000-BW) permit solid-phase permethylation and NaOH removal, all from an enclosed container. Reduce artifacts! Try our  Reactor columns today! The Nest Group, Inc. - "Not Just Columns ... Answers!" Learn more
Quick Poll

Data Integrity in the Lab
Data integrity in the laboratory is currently a hot topic. What is your company or laboratory doing about it? Take our quick poll today! The results will appear in an upcoming issue of LCGC magazine. Take the poll
Challenges and Benefits of Using SFC
Understand the value of SFC versus the drawbacks of what is available in the field today. This Q&A walks through a typical screening day and offers advice on what’s needed for an effective separations process. Read more
Featured Articles

New HPLC Systems and Related Products Introduced at Pittcon 2016: A Brief Review
Our annual review of new HPLC instruments and related products highlights the innovative features of new HPLC systems, modules, software, and product extensions.
Advances in Separations Technologies
Interested in mass spectrometry, chromatography, or electrophoresis? Looking for a conference with strong scientific content, robust program, world renowned speakers, poster sessions, courses, workshops, tutorials, panel discussions, major exposition showcasing new product launches? Check out HPLC 2016 San Francisco...largest, most recognized international conference and exposition devoted to advances in separations technologies, leading the way to ensure the separation sciences remain a powerful analytical tool. Learn more

How Does It Work? Part I: Pumps
Understanding how liquid chromatography pumps operate can help streamline solving pump problems.
The Role of Supercritical CO2 in Natural Product Research
Using supercritical CO2 in natural product research has many advantages whether it’s used for supercritical fluid extraction (SFE), characterization by analytical supercritical fluid chromatography (SFC), or isolation by preparative SFC.  Learn the theory and advantages of the supercritical process and compare them to the classic workflow. Learn more

On Maintaining Technical Proficiency
Maintaining and growing technical competencies requires effort. For scientists in any field, staying current with new developments is essential. The irony is that today, when we are flooded with information, keeping up with what matters is actually a bigger challenge than it was in the past.
 
Featured Application Note
EPA Method 544: Determination of Microcystins and Nodularin in Drinking Water by SPE and LC-MS/MS Detection
Microcystins are a group of hepatotoxins produced by freshwater cyanobacteria (also known as blue-green algae). Microcystins can be generated in large quantities during algae blooms and can pose a major risk to surface, ground and drinking water. This application note outlines a SPE method for the determination of 6 microcystins and nodularin in drinking water according to EPA Method 544. A 500 mL water sample is passed through a 6 mL UCT Enviro-Clean® SPE cartridge containing 150mg of divinylbenzene (DVB) based sorbent to extract the analytes. The final purified extract is then analyzed by LC-MS/MS equipped with a Selectra® C8 HPLC column. Learn more
 
Featured Products
New thermal desorption instruments Drug-to-Antibody Ratio (DAR) Analysis
Markes’ new ‘xr’ series of thermal desorption instruments pre-concentrate organic vapours sampled using sorbent tubes, canisters and on-line air streams, and as well as options for automation, incorporate powerful new advances that extend the capabilities of analytical laboratories analysing VOCs and SVOCs in air and materials.
Learn more
TSKgel Butyl-NPR, 2.5 µm HIC columns are optimized for the successful separation of unconjugated and drug conjugated mAb samples with baseline resolution. This enables an easy integration and quantification of different drug pay loads in ADC characterization. Learn more
 
New Webcasts
High-Resolution Quantitative Characterization of Intact Biopharmaceuticals and Their Proteoforms
Wednesday, May 25, 2016
8 am PDT | 11 am EDT | 4 pm BST | 5 pm CEST

The Main Causes of Laboratory Water Contamination and Strategies to Avoid Them
Tuesday, June 14, 2016
8 am PDT | 11 am EDT | 4 pm BST | 5 pm CEST

Pesticide Residue Analysis Webinar: GC–MS-MS-Based Multi-Residue Determination of Pesticides in Difficult Matrices
Wednesday, July 13, 2016
8 am PDT/ 10 am CDT/ 11 am EDT/ 4 pm BST/ 5 pm CEST
 
On Demand Webcasts