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LCGC North America | e-Separation Solutions
May 14, 2015
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The LCGC Blog
Troubleshooting Retention Time Issues in Gradient HPLC
While some of the relationships between parameters in gradient HPLC are complex, others are reasonably straightforward. Here, Tony Taylor explains, through an example, how adjusting the flow rate can improve resolution between critical peak pairs.
ISPPP2015
Don’t miss cutting-edge presentations delivered by leading scientists at the international conference on the Separation, Purification, and Characterization of Biologically Important Molecules. Learn about high speed, comprehensive, and ground breaking aspects of separation science as applied to classes of biochemical analyses and the consequences of these analyses; and latest innovations in technologies critical in clinical and biopharmaceutical research. July 29-31 in Philadelphia, PA, USA www.ISPPP.org
LCGC TV

Tips for Analyzing Three Types of Biological Fluids
From his work analyzing estrogen and other biological samples, Kevin Schug offers advice for dealing with the particular interferences of three different biological fluids: plasma, urine, and cerebrospinal fluid. He also offers advice for scientists who want to begin using 2D LC–MS-MS.
NEW: The 2015 Pharmaceutical reference materials catalogue from LGC
From Atorvastatin to Zopiclone: the new Pharmaceutical reference materials catalogue from LGC is now available. LGC now offers over 3,500 impurity and drug substance reference standards, and a selected range of primary standards produced under ISO Guide 34, which fulfill ICH, FDA and other regulatory body requirements. All products in the catalogue are linked directly to the LGC webshop for easy online ordering. Download catalogue
Featured Articles

The Simple Use of Statistical Overlap Theory in Chromatography
The statistical overlap theory of chromatography relates the number of peaks that appear in a chromatogram to the number of detectable components and the peak capacity. How can it be applied to chromatography in everyday usage?
Micro-SPE Desalting With Reduced Losses
New! Micro-volume TARGA® C18 SPE chemistries to desalt peptide, glycopeptide or glycan samples. Desalting with the water wettable, TARGA® C18 UltraMicroSpin™ columns, by loading in 100% water, improves recovery of these polar molecules prior to LCMS. Try our TARGA C18 as your desalting protocol, today! The Nest Group, Inc. - “Not Just Columns ... Answers!”

Enhanced-Fluidity Liquid Chromatography: Connecting the Dots Between Supercritical Fluid Chromatography, Conventional Subcritical Fluid Chromatography, and HPLC
In enhanced-fluidity LC, a dissolved gas, such as carbon dioxide, is added to the mobile phase. The resulting lower viscosity of the mobile phase and the increased diffusivity decreases analysis time and often improves efficiency.
PREP 2015 – Major conference on Preparative and Process Chromatography
Check out the outstanding program packed with papers on biochromatography, downstream processing, QbD, monoclonal antibodies, plasmids, enzymes, vaccines, VLPs, and other biopharmaceuticals, chiral molecules, SFC, fine chemicals, peptides, proteins, SMB, APIs, natural products, monoliths and new stationary phases, and more! Most recognized international conference showcasing the very latest scientific advances in Preparative and Process Chromatography. PREP2015, July 26-29 in Philadelphia, PA, USA www.PREPsymposium.org
Featured Products
Speed up your HPLC separations The First SEC-MALS detector for UHPLC Restek’s EZGC® Online Suite
Chromolith HPLC columns provide excellent separations in a fraction of the time that a standard particulate column will take. This enables higher speeds and higher sample throughput at lower back pressure. Learn more The mDAWN™ is the world's first multi-angle light scattering (MALS) detector that can be coupled to any UHPLC system in order to determine absolute molecular weights and sizes of polymers, peptides, and proteins.
Learn more
Speed Up Method Development With Restek’s EZGC® Suite. These tools will save you hours of calculations and guesswork. Free, web-based applications help you create model chromatograms, get column recommendations, translate methods and calculate flows! Learn more
New Webcasts

Therapeutic Protein Separations Using Diamond-Based Wide-Pore HPLC Columns
Wednesday, May 27, 2015
10:00 am PDT/ 10:00 am CDT/ 1:00 pm EDT



A Faster, More Sensitive Sample Preparation Solution for Bioanalytical Samples
Tuesday, June 23, 2015
8:00 am/ PDT/ 10:00 am CDT/ 11:00 am EDT