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The LCGC Blog |
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Troubleshooting Retention Time Issues in Gradient HPLC
While some of the relationships between parameters in gradient HPLC are complex, others are reasonably straightforward. Here, Tony Taylor explains, through an example, how adjusting the flow rate can improve resolution between critical peak pairs. |
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ISPPP2015 |
Don’t miss cutting-edge presentations delivered by leading scientists at the international conference on the Separation, Purification, and Characterization of Biologically Important Molecules. Learn about high speed, comprehensive, and ground breaking aspects of separation science as applied to classes of biochemical analyses and the consequences of these analyses; and latest innovations in technologies critical in clinical and biopharmaceutical research. July 29-31 in Philadelphia, PA, USA
www.ISPPP.org |
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LCGC TV |

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Tips for Analyzing Three Types of Biological Fluids
From his work analyzing estrogen and other biological samples, Kevin Schug offers advice for dealing with the particular interferences of three different biological fluids: plasma, urine, and cerebrospinal fluid. He also offers advice for scientists who want to begin using 2D LC–MS-MS. |
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NEW: The 2015 Pharmaceutical reference materials catalogue from LGC |
From Atorvastatin to Zopiclone: the new Pharmaceutical reference materials catalogue from LGC is now available. LGC now offers over 3,500 impurity and drug substance reference standards, and a selected range of primary standards produced under ISO Guide 34, which fulfill ICH, FDA and other regulatory body requirements. All products in the catalogue are linked directly to the LGC webshop for easy online ordering.
Download catalogue |
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Featured Articles |
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Micro-SPE Desalting With Reduced Losses |
New! Micro-volume TARGA® C18 SPE chemistries to desalt peptide, glycopeptide or glycan samples. Desalting with the water wettable, TARGA® C18 UltraMicroSpin™ columns, by loading in 100% water, improves recovery of these polar molecules prior to LCMS.
Try our TARGA C18 as your desalting protocol, today! The Nest Group, Inc. - “Not Just Columns ... Answers!” |
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PREP 2015 – Major conference on Preparative and Process Chromatography |
Check out the outstanding program packed with papers on biochromatography, downstream processing, QbD, monoclonal antibodies, plasmids, enzymes, vaccines, VLPs, and other biopharmaceuticals, chiral molecules, SFC, fine chemicals, peptides, proteins, SMB, APIs, natural products, monoliths and new stationary phases, and more! Most recognized international conference showcasing the very latest scientific advances in Preparative and Process Chromatography. PREP2015, July 26-29 in Philadelphia, PA, USA
www.PREPsymposium.org |
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Featured Products |
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Speed up your HPLC separations |
The First SEC-MALS detector for UHPLC |
Restek’s EZGC® Online Suite |
Chromolith HPLC columns provide excellent separations in a fraction of the time that a standard particulate column will take. This enables higher speeds and higher sample throughput at lower back pressure.
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The mDAWN™ is the world's first multi-angle light scattering (MALS) detector that can be coupled to any UHPLC system in order to determine absolute molecular weights and sizes of polymers, peptides, and proteins.
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Speed Up Method Development With Restek’s EZGC® Suite. These tools will save you hours of calculations and guesswork. Free, web-based applications help you create model chromatograms, get column recommendations, translate methods and calculate flows!
Learn more |
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