The LCGC Blog

Ghost Peaks in Gradient HPLC

I guess we have all had the issue at some time or other. A blank injection of eluent (or a zero volume dummy injection) gives rise to discreet peaks on the chromatographic baseline which may interfere with analyte peaks or increase the complexity of the chromatogram obtained, especially when performing trace analysis.chromatogram obtained, especially when performing trace analysis. Of course this is an undesirable situation and one which can be difficult to understand or troubleshoot as, if we didn't inject anything, how on earth do we end up with discrete beaks in the blank chromatogram? The secret to understanding the phenomenon lies in understanding the mechanisms of gradient of HPLC. Read more

Enter to Win & Save on Charge Variants Profiling Kits

Understanding charge variance of biopharmaceuticals or biosimilars is critical to ensure drug efficacy and safety. We've made this process easier and faster for you with our patented Thermo Scientific CX-1 pH Gradient Buffer Kits. Enter our competition to win a kit AND get a discount for the kits! Save Now.

LCGC TV

Quality by Design and Uncertainty and the Analytical Target Profile in QbD

By establishing criteria for measurement uncertainty, the rest of the method development process can be guided. Kimber Barnett from Pfizer discusses how a QbD approach to chromatographic method development can improve our understanding of the uncertainty of analytical results. She also highlights the importance of the analytical target profile and how to determine it. Read more

Increase Throughput for EPA Methods 625 and 8270

Running the required quality control samples can consume a significant portion of a laboratory's 12-hour shift. This paper describes how combining fast GC with the rapid mass spectral scanning and data acquisition capabilities of Shimadzu's GCMS-QP2020 will shorten analysis times, thereby increasing a lab's productivity, while providing results that meet or exceed EPA Method requirements. Learn more

Featured Article

Maximization of Selectivity in Reversed-Phase Liquid Chromatographic Method Development Strategies

The approach described here will help chromatographers produce highly efficient method development strategies for reversed-phase LC separations in a relatively short time frame. Read more

New Topaz Inlet Liners

Topaz inlet liners deliver inertness you'll have to see for yourself to believe. The new deactivation technology means unbelievably low breakdown for accurate and precise low-level GC analyses. Unbeatable manufacturing and QC ensure superior reliability across compound classes. And unparalleled cleanliness maximizes GC uptime and lab productivity. Step away from the frustration and uncertainty of ordinary liners—and expect more, every time, with new Topaz GC inlet liners. Learn more