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| The LCGC Blog |
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Column Selection for HPLC Method Development
Are we making the most of the ToolKit method development platforms approach? The toolkit approach should provide a highly efficient and wide ranging exploration of the separation possibilities for the application under investigation, in particular the selectivity, which will drive the separation under development. |
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| The HPLC 2016 Previews—Trends In Separation Science |
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Why Effective HPLC Separations Are Critical for the Analysis of High-Complexity Systems
In proteomics studies, proteins are digested into hundreds of thousands of peptides, thus creating very large and complicated mixtures. Simultaneous electrospray ionization of these complex mixtures, however, results in suppression of ion formation. Therefore, it is essential to have effective chromatographic methods to separate the peptides before analysis with mass spectrometry, to relieve ion suppression and to allow the mass spectrometer sufficient time to collect tandem mass spectra of peptide ions. The challenges involved in developing such separations are great, however.
This is the first of a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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| LCGC TV |
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The Funding Environment in Europe Compared to the US
Changes in the focus of US funding agencies have made life harder for many separation scientists. Apryll Stalcup of the Irish Separation Science Cluster discusses the differences between research funding in Europe and the US, and the variety of mechanisms and incentives in Europe that foster the separation sciences. |
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Take Our Quick Poll!
What problems have you faced with gradient methods? And how about isocratic methods? Which one is the biggest? Please share your experiences by taking this quick, three-question poll. The results will appear in an upcoming issue of LCGC magazine. |
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| Application Note |
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Featured Products
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| High Pressure UHPLC Tubing |
Easy Method Transfer |
IDEX Health and Science offers a line of robust high-pressure tubing - designed specifically for use in high-pressure and very high-pressure applications such as UHPLC. Explore  UHPLC tubing! |
TSKgel UP-SW3000, 2 µm SEC columns are designed for the analysis of monoclonal antibodies and other biopharma products. They are compatible with both HPLC and UHPLC systems for ease of method transfer.  Learn more |
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| Parker |
UHPLC columns speed at highest performance |
Parker hydrogen generators are designed to replace high pressure cylinders. Gas generators from Parker provide consistently high purity gas at safe, low pressures. Most Parker hydrogen generators have a payback period of less than two years.
 Learn more |
Using UHPLC methods with short columns, narrow inner diameters and small particles sizes, it is possible to speed up analyses up to ten-fold.
Purospher® STAR RP-18 endcapped UHPLC columns packed with 2 µm silica sorbent can decrease separation time by a factor of 10 and reduce solvent consumption by up to 90%.
They are therefore ideal for  ultra-fast applications such as high-throughput screening and QC analyses, process monitoring and method development.  Learn more |
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| CXT750 Automated Frozen Sample Aliquotter |
Jasco |
Extracts multiple frozen cores, maintaining parent sample and frozen aliquots at -80°C. Eliminates freeze-thaw cycles. Preserves sample integrity, enabling LC/MS-MS analysis of labile compounds and improving accuracy of quantitative bioanalysis.
 Learn more |
The new LC-4000 series does HPLC your way. Available in three customizable configurations that adapt to your lab's requirements, the LC-4000 series allows you to take advantage of a wide variety of column technologies.
 Learn more |
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New Webcasts
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On Demand Webcasts
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