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Featured Interview
Ron Majors is often one of the first names chromatographers come to know during their education, and he is quite famous in the chromatography community. Here, he discusses his humble beginnings, career highlights, and more.
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The LCGC Blog
Several years ago, I would have held the stance that environmental analysis was fairly boring. How complicated can water be? I am not ashamed to say that was a naïve view. It is clear from our research and related research by others on similar topics that much more work in these areas is needed. Standard methods cannot solely accommodate the growing list of targets and the multitude of unknowns associated with complex samples taken from the interface between the petroleum industry and the environment.
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Size exclusion chromatography (SEC) enables scientists to conduct innovative research and accelerate drug discovery. Sorbtech’s SEC protein and nucleic acid purification products provide greater confidence throughout development and manufacturing. Choose from a range of our high-end, affordably priced size exclusion solutions, which include dextran based SorbaDex™, agarose based SorbaRose™, and SuperSorb™—an agarose-dextran matrix. Find out more at  www.sorbtech.com, or call us at 770-936-0323.
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Featured Articles
There is a resurgence of interest in chemical derivatization schemes in separation science, particularly in sample preparation. Here’s why. Read more
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The name PULVERISETTE by FRITSCH is synonymous with fast, loss-free fine grinding of samples. Consistent reproducibility for analytical accuracy in sample preparation. Achieve fine grinding of a wide range materials. Perform dry, in suspension, or in inert gas. PLANETARY BALL MILLS perform comminution, mixing, and homogenizing of solids, emulsions, and pastes.  Learn more
Paying attention to the details of mobile-phase preparation can have a big impact on the reproducibility of HILIC separations. Read more
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A look at techniques for charge-variant analysis of monoclonal antibodies and the question of whether pH gradients are really better than salt gradients Read more
Part I of this series on practical perspectives of data integrity focuses on sample management, transport, and preparation. Read more
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In many stages of mAb development, harvest cell culture samples must be screened for their IgG titers. TSKgel Protein A-5PW is specifically designed for the fast analysis of mAb concentration in cell culture supernatant. Its wide dynamic range allows equally accurate determination of comparatively low mAb titers in early development and high mAb titers of optimized cell lines in production.  Learn more
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Application Note
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Patulin is a naturally occurring mycotoxin that is produced by several species of fungi, such as Aspergillus, Penicillium and Byssochlamys. Typically growing on fruit, such as apples, it has recently been implicated as a possible carcinogen and teratogen, although an official designation has not yet been made. The following detailed method utilizes a QuEChERS approach coupled with UHPLC-MS/MS for processed foods.
• QuEChERS extraction procedure for the analysis of patulin in processed foods.
• The inclusion of graphitized carbon black (GCB) in the dispersive-SPE (dSPE) cleanup step produces a clean sample extract.
• Mean analyte recovery was 86.6% for samples fortified at 50 µg/kg.
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