The LCGC Blog
Virtually exclusively, liquid chromatography–mass spectrometry (LC–MS)–based assays for protein quantitation rely on bottom-up strategies, where the protein is initially digested into constituent peptides during sample preparation. Top-down intact protein quantitation, especially using affordable, low resolution triple quadrupole (QQQ) mass spectrometers, has been largely unexplored.
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Restek has expanded its line of LC accessories to bring you even more of what you need to keep your LC flowing. Browse top-quality couplers, fittings, unions, tees, and crosses; PEEK and stainless steel tubing; mobile phase maintenance and safety products including bottle tops, valves, filters, and spargers—and that's on top of our already wide selection of instrument replacement parts and supplies. Don't stop at the column; outfit your entire LC system with Restek LC products.
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Articles
The elution mechanisms for gradient and isocratic HPLC are fundamentally different from each other. Here is what you should understand about both.
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Incorporating core-shell technology, Shimadzu's Velox HPLC columns enable users to achieve increased separations and faster analysis times on any LC platform, while providing excellent lifetime for the most challenging sample matrices. Utilize Velox columns and achieve improved separation and detection, outstanding analysis and data integrity, reduced analysis costs, higher productivity, and increased sample throughput.  Learn more
We look at problems in LC that can be traced to contaminated solvents or reagents, and how sample solvent effects can lead to poor performance in HILIC.
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There are many factors to consider in a data integrity and governance program. Fortunately, a simple diagram can help us understand what needs to be covered.
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The occurrence of additives like plasticizers in plastic food packaging has been documented, but information on their potential migration into foods is limited. In this pilot study, 2D GC–MS was used to identify chemicals extracted from a common stretch plastic film with a series of organic solvents. In total, 91 compounds were tentatively identified, including five PCBs.
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The TSKgel FcR-IIIA-NPR is the world's first FcRγ affinity chromatography analysis column for the analysis of IgG glycoforms. The stationary phase utilizes a recombinant human Fcγ receptor IIIa as a ligand bound to a nonporous polymethacrylate polymer. The resulting elution profile of the glycoprotein mimics antibody-dependent cellular cytotoxicity (ADCC) activity, which is correlated to the composition of the N-glycans.  Learn more
Incognito recalls the words of wisdom from an "old-school" supervisor who mentored him on the art of effective trace analysis.
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