LCGC North America Application Note Alert Having trouble viewing this email? Click here IEX-WCX Charge Variant Analysis of Trastuzumab Ion-exchange chromatography for charge variant analysis of trastuzumab under pH and salt gradients using a bioZen™ 6 μm WCX column. Neutral and Sialylated Glycans by HILIC and SPE Quantitation of neutral and sialylated glycans of glycoproteins using bioZen HILIC chromatography and solid-phase extraction (SPE) mAb Characterization: New bioZen LC Column Portfolio Designed for the analysis of protein charge variants, aggregates, fragments, released glycans, peptide mapping, intact mass, peptide quantitation, and drug antibody ratio. N-Glycan Clean-Up with Microelution HILIC SPE Sample clean-up after N-linked glycan release and labeling using biozen N-glycan clean-up HILIC solid-phase extraction microelution 96-well plate. New Bioinert Titanium HPLC/UHPLC Hardware Titanium-infused biocompatible hardware and frit doesn’t interfere with protein or peptide integrity and curtails unwanted secondary interactions between injection and detection. Aggregate Analysis with bioZen SEC Columns BioZen SEC columns were developed with a combination of UHPLC efficiency and higher sensitivity, to drive resolution and identification of even lower level mAb aggregates. Average DAR for Cysteine Conjugates Average DAR is a critical attribute for antibody-drug conjugates (ADCs) and reversed-phase LC becoming a preferred route for analysis.