LCGC North America Application Note Alert Having trouble viewing this email? Click here HPLC Analysis of Apramycin Sulfate Post-column derivatization with Ninhydrin reagent for analysis of 3-hydroxyapramycin, lividamine, caerulomycin, 2-deoxystreptamine as impurities in Apramycin Sulfate. Peptide Analysis with 1.6 µm Columns The Develosil UHPLC series gives sufficient retention and separation while using ammonium formate for the mobile phase; it can produce useful results in LC–MS. Fast Separation of Triptans in Rat Plasma on ZirChrom®-PBD The following reviews a published comparison of the ZirChrom®-PBD column to the HypersilTM BDS C18 column for the analysis of triptans in rat plasma. Guide for Successful (U)HPLC Method Transfer Method transfer is a common practice in HPLC. Read about instrumental parameters, challenges, and solutions to consider during the transfer of an analytical HPLC method.   LC-MS Reduce Peak Dispersion in HPLC/UPLC Set-Ups Post-column flow splitting can cause excessive peak broadening and reproducibility issues for LC–MS scientists, but this can be solved using modern splitting technology. Metal-Free Columns for Troubleshooting Phosphorylated Compounds Phosphorylated compounds adsorb to stainless steel, negatively impacting peak shape, sample recovery, and sensitivity. Our metal-free columns can improve analysis. Simultaneous Analysis of Catecholamines and Metanephrines in Urine by LC-MS/MS Analysis of these polar compounds using reversed-phase LC can be difficult due to limited chromatographic retention, which results in poor separation of the analytes from closely eluting matrix interferences.   SEC Identifying Short-Chain Branched Polymers Short- and long-chain branching in polymers are distinguished and quantified by SEC coupled to multi-angle light scattering and differential viscometry detectors.