LCGC Europe Application Note Alert Having trouble viewing this email? Click here Differentiating Short- from Long-Chain Branching Short- and long-chain branching in polymers are distinguished and quantified by SEC coupled to multi-angle light scattering and differential viscometry detectors. UHPLC-SEC with Absolute Molar Mass UHPLC separations of proteins and biopolymers benefits from the addition of multi-angle light scattering for the determination of absolute molar mass and size. ADC Drug-Antibody Ratio Analyzed by SEC-MALS Drug-antibody ratio (DAR), a critical attribute of antibody-drug conjugates (ADC), can be determined by adding multi-angle light scattering to SEC (SEC-MALS) Understanding Antibody and Viral Glycoprotein Interactions Viral glycoproteins (vGP) and their interactions with neutralizing antibodies are well-characterized by multi-angle light scattering (MALS) VLP Characterization - the Light Scattering Toolbox Virus-like particle therapeutics require a unique set of characterization tools for each stage of the R&D pipeline. Light-scattering-based techniques are key. Heparin Charge-Mass Ratio Multi-angle light scattering (SEC-MALS) provides absolute molar mass of heparin without calibration standards, while charge from PALS indicates adulterations. Enzymatically Cross-Linked Casein Characterized by FFF-MALS Cross-linking of casein by mTGase to produce textured dairy products affects molecular conformation and density. These properties are characterized by SEC-MALS-DLS. WP9001 Nanoparticle Characterization with the Light Scattering Toolkit A suite of integrated separation, isolation, and characterization tools overcome the challenges of nanoparticle characterization. High-Speed Process Monitoring with UHP-SEC-MALS UHP-SEC with multi-angle light scattering (µSEC-MALS) is a powerful tool for process and stability testing, to monitor the formation of mAb aggregates and fragments.