Copolymers cannot be characterized by standard GPC. A triple-detection method, incorporating MALS, UV, and RI measurement is shown to provide complete characterization.
This white paper explains how MALS and intrinsic viscosity detectors, coupled to SEC or field-flow fractionation, are used to fully characterize branched polymers.
UHP-SEC offers major advantages over HP-SEC in terms of shorter run times and reduced consumption of sample and solvent. µSEC-MALS transitions the benefits of absolute molar mass and size characterization of synthetic polymers to UHP-SEC.
SEC combined with multiangle light scattering and intrinsic viscosity enables absolute determination of the molecular weight, size, and conformation of hyaluronic acid.
Sodium alginate is a food thickening agent that may be irradiated for sterilization. In this note, changes in biopolymer molar mass and conformation which impact macroscopic properties are analyzed by SEC-MALS.
Host-guest complexes constitute transient conjugates that are quite stable due to mechanical incorporation of the guest into the hollow host molecule. MALS and AF4 determine the heteroternary stoichiometry and stability of such a complex.
Self-association is crucial for regulation of certain kinase proteins. However, a kinase fragment that lacks the oligomerization domain is still dimeric in solution, as determined by SEC-MALS.
This application note demonstrates how different insulin formulations are characterized by SEC coupled to MALS for absolute determination of molar mass and oligomer distributions, including dynamic equilibria.
MALS coupled to SEC provides a fast, accurate means of characterizing reagents for ligand-binding assays to assess aggregation which adversely impacts ELISA results.
