LCGC Europe Application Note Alert Having trouble viewing this email? Click here Molecular Weight of Cellulose in DMAC Unmodified cellulose can be dissolved in DMAC, but without suitable calibration standards cannot be analyzed by GPC. SEC-MALS with a DAWN overcomes this obstacle. Chitosan Molar Mass Determination Biopolymers such as chitosan may exhibit short-chain or long-chain branching. Regardless of conformation, SEC-MALS provides absolute molar mass distributions. Analysis of LMW Heparin Molar mass analysis of low-molecular-weight heparin is readily performed by SEC-MALS without standards. The results are comparable to analysis per the EP monograph. Stoichiometry of Disordered Protein Complexes Non-globular proteins elute earlier on SEC than protein standards of the same molar mass. SEC-MALS permits analysis of such proteins and their high-order complexes. Characterization of Protein PEGylation Processes PEGylated proteins are characterized by SEC-MALS to determine protein-PEG ratio and the molar masses of the constituent protein and PEG at each eluting fraction. Characterization of Peptides by SEC-MALS SEC-MALS is applicable to low molar mass macromolecules as well as proteins and polymers. Here peptides of 1000 g/mol and below are robustly analyzed online. Characterization of Multi-Protein Complexes SEC-MALS analyzes multi-protein complexes in order to determine total molecular weight and absolute stoichiometry (number of each type of molecule in the complex). Molar Mass of Fluorescent Lignosulfate Polymers GPC column calibration standards are not available for lignin, which is often branched. Analysis by SEC-MALS requires corrections for laser absorption and fluorescence. Analysis of Linear and Branched Amylose Branching analysis of amylopectin requires a linear amylose analog. Synthetic amylose was prepared and characterized by SEC-MALS to determine if branching is present. Protein Characterization by IEX-MALS IEX-MALS overcomes some of the restrictions of SEC-MALS that arise from SEC, while providing absolute molar mass, size and quality assessment of heterogeneous proteins.