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This article describes how size-exclusion chromatography (SEC) combined with high-resolution mass spectrometry (HRMS) can be applied to the detailed characterization of monoclonal antibodies (mAbs) and antibody–drug conjugates (ADCs). Read more
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The DynaPro Plate Reader III from Wyatt Technology is an integrated instrument for automated, high-throughput characterization of size, molecular weight, aggregation, and stability of macromolecules and nanoparticles. The instrument, which combines both dynamic light scattering and static light scattering approaches, contains a single, fully automated platform with measurements taken directly in 96, 384, or 1536 microwell plates.
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Features
A simple and robust size-exclusion chromatography (SEC) method has been developed for characterizing a multimeric PEG–protein conjugate. A wide range of size variants of the conjugate, ranging from 50 kDa to >1000 kDa, can be resolved and quantitated. Read more
Long-term column use can lead to on-column methionine oxidation during liquid chromatography–tandem mass spectrometry (LC–MS/MS) peptide mapping of antibody-based biotherapeutics. Following the approach described here minimizes the risk of measuring oxidative artifacts, and helps generate high quality data to provide reliable quantitative information about product-related heterogeneities. Read more
Advanced separation and mass spectrometry methods enable comprehensive profiling of the inherent glycan heterogeneities of protein therapeutics. In particular, reversed-phase high performance liquid chromatography (HPLC)-based multiattribute methods (MAMs) provide a wealth of information, and other techniques, such as hydrophilic interaction liquid chromatography (HILIC) and capillary electrophoresis-mass spectrometry (CE–MS), also continue to evolve.
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Application Note
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This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.
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FEATURED PRODUCT
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The DynaPro Plate Reader III from Wyatt Technology is an integrated instrument for automated, high-throughput characterization of size, molecular weight, aggregation, and stability of macromolecules and nanoparticles.
 Learn more
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