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November 23, 2015 Follow us on:

Chromatography Corner The Evolution of UHPLC in Pharmaceutical Analysis Michael Dong offers advice on using ultrahigh-pressure liquid chromatography (UHPLC) for method development in pharmaceutical analysis and the important distinction between method conversion and method transfer. Chromatography News Image credit: MOLEKUUL/SCIENCE PHOTO LIBRARY/Getty Images High-Throughput Screening of Heparin Using LC–MS Researchers have published a new approach to heparin screening that allows the detection of adulteration within one hour. Published in the journal Analytical Chemistry, the study presents a screening strategy using hydrogen peroxide digestion followed by fast reversed-phase ion pairing liquid chromatography (reversed-phase IP–LC) coupled with tandem mass spectrometry (MS–MS) to detect contamination of heparin samples. Column selection makes all the difference in chromatography. Get the answers you need by choosing the most appropriate analytical, high throughput, HPLC, UHPLC, or bioLC column for your application with the Column Genie app. It was built with the goal of enabling you to search by chromatographic mode and application area, as well as giving access to full column specifications, application notes and technical guides. Learn more Featured Article Image credit: Courtesy of the authors Removal of Contaminant Peaks in Reversed-Phase Gradient Liquid Chromatography for Improved Detection of Pharmaceutical Impurities This article presents a method for comparing the levels of baseline interference arising from common laboratory mobile phase contamination sources and assesses different approaches for removing dissolved contaminants to generate interference-free chromatogram baselines. The authors demonstrate that recirculating mobile phase through a semi-preparative scale column using a reagent delivery pump has advantages over previously published mobile phase decontamination methods. Separation Science in Drug Development, Part III: Analytical Development The third installment in this series provides an overview of modern practices of separation science in small-molecule drug development. It highlights approaches in high performance liquid chromatography (HPLC) method development and physical or chemical characterization to support process chemistry and formulation development, and for assessment or control of clinical trial materials.   Featured Products LC column selector app New Thermo ScientificTM Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer Get the answers you need by choosing the most appropriate analytical, high throughput, HPLC, UHPLC, or bioLC column for your application with the new Column Genie app. Learn more Test new limits of detection, characterization and quantitation with the latest TribridTM mass spectrometer. The new Thermo ScientificTM Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer is designed to expand performance in advanced proteomics, biopharma and metabolomics applications, including quantitation using isobaric tags, low level PTM analysis, data independent acquisition (DIA), and top down proteomics. The new instrument features enhanced sensitivity resulting in improved analyte detection, characterization and quantitation, enabling scientists to perform more comprehensive sample analyses faster and with better accuracy than ever before. Breakthrough Gains for Quantitative Biology Sensitivity Transformed. Learn More   On Demand Webcast Column Thermostatting—Understanding an Underestimated Parameter to Improve Efficiency and Selectivity in UHPLC