To fully characterize a protein biopharmaceutical, it must be broken down into smaller segments (peptides). Several HPLC techniques can be used to provide a wealth of information on everything from post-translational modifications to glycol profile to information on similarity when characterizing biosimilars.
The “greenest solution” is certainly using no solvent but GPC/SEC as a LC technique requires the use of a mobile phase. The growing awareness of the need for more sustainable (greener) solutions has focused attention on environmentally and health friendly solvents and solutions.
This article introduces the theory and application of a novel data analysis technique for PDA detection to accurately determine and quantify single compounds, even from overlapping peaks, without the need for MS detection.
The latest research news and news in brief are featured in this issue.
Visit CHROMacademy.com our new e-learning site dedicated to the analytical chemistry community.
Need innovative ideas or methods that are tried and true? Visit industrymatter.com for a collection of analyzed, valuable information that's worth a read.
The global digital publication for chromatographers
10 December 2019
