THE BAKER’S DOZEN: THE TOP 13 ARTICLES OF 2019

With the arrival of the new year, what better time to reflect on 2019 and look back at some of the most popular articles from LCGC Europe and The Column that attracted readers' attention last year? These articles illustrate the wide range of content we provide offering practical advice, technical expertise, unique insights, and the latest trends in separation science.

4. Denaturing and Native Size-Exclusion Chromatography Coupled to High-Resolution Mass Spectrometry for Detailed Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates

This article describes how size-exclusion chromatography (SEC) combined with high-resolution mass spectrometry (HRMS) can be applied to the detailed characterization of mAbs and ADCs. Read more

5. Strategies for the Quantification of Endogenously Present Small Molecules in Biological Samples

The main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of MS in the field of bioanalysis, a special focus will be placed on quantification by LC–MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided. Read more

6. Clinical Metabolomics: Expanding the Metabolome Coverage Using Advanced Analytical Techniques

This review article discusses the novel separation and detection strategies that are considered promising in clinical metabolomics to enhance the metabolome coverage. It includes HILIC, SFC, multidimensional LC approaches, as well as IM-MS and DIA analysis methods. Read more

7. Online LC–GC: The Ultimate “GC Connection”

In this instalment of “GC Connections”, the advantages of multidimensional chromatography with HPLC as the first dimension and GC as the second are discussed. Read more

8. Methods from Mars? Coping with Chromatographic Legacies

Sometimes, the conditions specified in legacy liquid chromatography (LC) methods seem like they come from a different planet. In this instalment of “LC Troubleshooting”, we looked at which conditions to keep, and which ones to let go of. Read more

9. Ion Mobility–Mass Spectrometry for Food Analysis: An Update

In this article, the potential of ion mobility–mass spectrometry (IM-MS) to increase selectivity and for additional identity confirmation is investigated. An extensive evaluation of IM-MS instruments was performed on a broad test set of food safety contaminants. Read more

10. New Liquid Chromatography Columns and Accessories for 2019

Our annual review of new liquid chromatography (LC) columns and accessories, introduced at Pittcon and other events. Read more

11. New Sample Preparation Products and Accessories

This yearly report on new products introduced since March 2018 covers sample preparation instrumentation, supplies, and accessories. Read more

12. A Design for Life Sciences

The Column spoke to Robert Shaw, Steph Turnbull, and Sophie Bailes from AstraZeneca about their work in quality by design (QbD) in the pharmaceutical industry. Read more

13. Tips to Boost Your Trace Analysis Skills

Incognito recalls the words of wisdom from an “old-school” supervisor who mentored him on the art of effective trace analysis. Read more

Agilent 6495C Triple Quadrupole LC/MS system

The highest performance LC/TQ available with Agilent's third generation iFunnel design, ideally suited for peptide quant as well as multiresidue applications that require ppt sensitivity. The combination of utmost sensitivity, extended mass range, ease of maintenance with VacShield technology, and the power and flexibility of MassHunter makes this the system of choice for demanding applications./ Learn More /

Chromatographic Method Modelling Software

Based on fundamental scientific theories such as the solvophobic interactions and linear solvent strength model, DryLab is an industry-standard chromatographic method modelling software package for (U)HPLC method development and optimization. / Learn More /

PharmaFluidics’ µPAC™ Family

While our 200cm µPAC™ is your best choice for comprehensive proteomics, our 50cm µPAC™ is recommended for routine analyses. Furthermore, µPAC™ Trapping columns fulfill your needs for peptide sample enrichment. / Learn More /

A powerful new tool for the separation of charged species

The Postnova EAF2000 instrument using Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) technology allows the particle size or molar mass distributions to be further differentiated and transformed into charge distributions. /Learn more/

New Widepore Peptide/Protein Column

YMC-Triart Bio C18 offers 300 Å pore size and is designed for peptides, proteins, and oligonucleotides. This robust C18 phase provides a wide temperature and pH range for mid-MW biomolecules. / Read more /