LCGC Europe Application Note Alert Having trouble viewing this email? Click here mAb Method Using Isopropanol in HIC This application note shows how to achieve higher recoveries for commercially available mAbs using 15% isopropanol as modifier in HIC mode. Generic Analyses of mAbs with Reversed-Phase (U)HPLC This application note demonstrates how to achieve robust chromatographic results for commercially available mAbs with higher sensitivity and sharper peaks at 80 °C. SEC Analysis of mAb Fragments The analysis of intact mAbs and their fragments using the UHPLC-compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described. Reaction Monitoring of ADCs The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV–vis is shown. High Temperature Reversed-Phase Analyses of mAbs This application note shows how to achieve robust chromatographic results for two commercially available mAbs using a modern temperature-stable widepore reversed-phase phase. AEX Separation of Oligonucleotides A method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger, BioPro IEX QF. Challenging Analysis of Amyloid β (Aβ) Proteins The method development for the simultaneous determination of highly hydrophobic amyloid β peptides is described. Sensitive LC–MS/MS Analysis of Sphingophospholipids The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.