In our October issue:
COLUMNS:
LC TROUBLESHOOTING
Minimizing Fluctuating Peptide Retention in 2D-LC: How to Address a Moving Target
Retention of peptides is strongly dependent on solvent composition in reversed-phase separations with gradient elution. In this instalment we provide tips, tricks, and suggestions for best practices to help minimize retention time variations over time.
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COLUMN WATCH
Highlights from the HPLC 2019 Symposium
TThe 48th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2019), chaired by Alberto Cavazzini and Massimo Morbidelli, was held from 16–20 June in Milan, Italy. This instalment of "Column Watch" covers many of the highlights observed at the symposium. Read more >>
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BIOPHARMACEUTICAL PERSPECTIVES
Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry
This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry. Read more >>
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THE ESSENTIALS
What a GC–MS Tune Report Can Tell You
A tune report is invaluable because it indicates how well the instrument is operating, and is an essential tool when troubleshooting is required.
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| ANALYSIS FOCUS: |
Petroleum Pollution Analysis with Ramped Pyrolysis GC–MS
LCGC Europe spoke to Zhanfei Liu from the Marine Science Institute at the University of Texas at Austin, USA, on the role of chromatography in petroleum pollution analysis.
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| PEER-REVIEWED ARTICLE: |
Design of Experiment Strategy for an Electrospray Ionization Optimization in Supercritical Fluid Chromatography–Mass Spectrometry Coupling
The optimization of adjustable factors in electrospray ionization (ESI) by design of experiments (DoE) is presented. This approach allows a comprehensive and systematic optimization of all relevant factors for the ionization of compounds and increases the understanding about factor influences on the ionization efficiency.
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This issue of LCGC Europe also features the Advances in Food and Beverage Analysis supplement.
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