FEATURES

Making the Most of Size-Exclusion Bioseparations Using Sub-2-µm Column Technology

The fundamental aspects of achieving high performance using sub-2-μm size-exclusion chromatography (SEC) columns are described. In addition, trends in the biopharmaceutical industry are discussed, including challenges that can be addressed using modern size-exclusion technologies. Read more

Tackling Top-Down Protein Analysis

Kevin Schug discusses the advantages of using mobile phases with different pH values in the first and second dimension when using comprehensive two dimensional liquid chromatography (2D-LC) methods for top down protein analysis. Read more

Tips & Tricks GPC/SEC: Silica Versus Polymer-Based Columns

The chemistry of samples analyzed using gel permeation chromatography/size-exclusion chromatography/gel filtration chromatography (GPC/SEC/GFC) is very diverse. Different chemistries of stationary phases are required to allow for true size separation. Several types of materials are available, all of which have their advantages and limitations. While silica-based stationary phases are most common in HPLC, for macromolecules polymer-based phases are popular. Read more

Preview of Topics at HPLC 2020, Part 2

In this preview, Gert Desmet explores the possibilities and challenges of producing chromatographic media with two-photon polymerization printing. This is the second in a series of articles exploring topics that will be addressed at the HPLC 2020 conference in San Diego, from June 20 to 25. Read more

NEWS

Investigating the Environmental Impact of Anticoccidials

Researchers have developed a new liquid chromatography tandem mass spectrometry (LC–MS/MS) method for the simultaneous chromatographic separation and detection of anticoccidials, which are used as an agricultural animal treatment, in environmental waters. Read more