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| Bruker Daltonics, August 2019 |
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| In Depth Analysis of Host Cell Proteins from Antibody Preparations using PASEF |
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| In the rapidly expanding arena of biotherapeutic analysis and bioprocess development, the analysis of host cell proteins (HCPs) at the ppm level is critical. ELISA is currently the gold standard for QC applications but non-targeted methods such as mass spectrometry are now emerging. |
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| The advantages of mass spectrometry include rapid method development and identification of all detected proteins. The low abundance of HCPs still presents a significant challenge and initial work in this field has often required specialized setups. Download the application note to learn more on how PASEF (parallel accumulation and serial fragmentation) scans, as implemented on the timsTOF Pro QTOF, can be applied to HCP analysis using routine analytical, or nanoflow configurations to achieve the goal of sensitive detection with enhanced speed and data quality. |
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| What you will discover from the work performed on the timsTOF Pro QTOF: |
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PASEF scans improve the sensitivity of routine peptide mapping enabling the detection of HCPs at required sub 100 ppm levels |
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PASEF coupled to nanoLC facilitates detection of previously unreported trace level HCPs |
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The quality of MS/MS sequence spectra provided by the timsTOF Pro with PASEF allows high confidence in protein ID even with only 1-2 peptides |
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The depth of HCP identification provided by PASEF technology allows fingerprinting of biomanufacturing processes and ability toeasily identify the effects of changes in these procedures |
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