|
|
|
|
|
NEW efficient and robust nano- and capillary LC separations technique using micro Pillar Array Columns
As an alternative to the conventional packed bed nano LC columns that are frequently used in bottom-up proteomics research, a breakthrough in column manufacturing recently led to the introduction of silicon-based micromachined nano LC chip columns known as micro Pillar Array Columns (μPAC™) that offers the following benefits
Increased sensitivity due to high degree of order Unrivalled robustness and number of injections due to the solid silicon backbone Outperforming reproducibility due to the mask-based etching process Less column replacements due to the high number of injections Less prone to clogging due to high column permeability Sharper peaks and excellent separation power due to exceptional column length
|
|
|
After the introduction of a 200 cm long column, which is ideally suited to perform comprehensive proteome research, a 50 cm long μPAC™ column is also available which can be used in a more routine research setting. With an internal volume of 3 μL, this column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90 minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min.
|
|
|
|
Recently performed experiments with 500 ng of HeLa cell digest indicate that an increase in protein identifications up to 50% and a gain of 70% in peptide identifications can be achieved when comparing the 50 cm μPAC™ column to the current state-of-the-art packed bed columns.
|
|
|
Early this year, also a µPAC™ capLC column was launched that provides a versatile output in terms of separation performance and throughput. As a consequence of the low column back pressure, the same column can be operated over a range of flow rates (1-15 µL/min), enabling analytical scientists to tune LC methods according to the sample complexity or to the size of the sample set.
|
|
|
|
"Exchanging columns is a necessary evil [...]. However, the µPAC™ has been running for over five months continuously , taking several thousands of injections[...]."
Dr. Duncan Smith – Head of Mass Spectrometry
Visit our website to learn more!
www.pharmafluids.com
|
|
|
Message above is a paid advertisement. Content is provided by the sponsor. You received this email because you are a customer of BioPharm, an MJH Life Sciences™ brand. You are on the mailing list as %%Member_Addr%%.
|
|
|
|
