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COLUMNS
Column Watch: Method Translation in Gas Chromatography
Kenneth Lyman and Ron Majors
Recently, there has been a trend towards developing faster chromatographic separations to improve productivity. In GC, the emphasis is on decreasing the column diameter, shortening the column length, and using hydrogen as a carrier gas. To maintain separation integrity, there must be changes made in the method to keep the retention order and selectivity the same. The process of making these changes is called method translation. One can go through the calculations using basic chromatographic theory but it is much easier to use method translation software that allows the changes to be made more conveniently. This installment of Column Watch will discuss the necessary calculations and illustrate the use of the software (which is often free) to achieve method translations in GC.

LC Troubleshooting: Troubleshooting Basics, I: Where to Start
John W. Dolan
Where do you start when you encounter a new problem with your LC?

GC Connections: Valves for Gas Chromatography: Applications
John Hinshaw
In the second installment of this series, John Hinshaw discusses the application of standard gas switching valves for packed and micropacked column backflushing and sequencing.

Innovations in HPLC: The Evolution of Pulsed Electrochemical Detection Waveforms
Guest author: William R. LaCourse, Ph.D., Chair & Professor of Analytical Chemistry, University of Maryland, Baltimore County
The article reviews pulsed electrochemical detection (PED) waveforms from their historical beginnings to the ones commonly used today. It then addresses the failures of the method and discusses the applications and ideas that are up and coming.

ARTICLES
SAMPLE PREP
Improving the Efficiency of Fatty Acid Methyl Esters Preparation Using Automated Sample Preparation Techniques
Rebecca A Veeneman, PhD, R&D Chemist, Agilent Technologies
An automated method for esterifying fatty acids in canola oil samples is presented. Using an automated sample preparation instrument, a side-by-side comparison was undertaken comparing a method for the automated system adapted from a previously published method to an analogous method performed manually. Overall, the automated method resulted in better precision and accuracy with smaller amounts of reagents used and less time required from the operator to complete the task.

LC–MS-MS
Determination of Clenbuterol-like Beta-agonist Residues in Hair
Wan-hua YANG, Department of Pharmacy, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai China; and Wen-ting LING, Qin Feng, and Gui-liang CHEN, all from the Shanghai Institute for Food and Drug Control, Shanghai China
All beta-adrenoceptor agonists (ß–agonists), such as clenbuterol, are currently banned from use in edible animals in China, USA, and the EU. However, there have been few studies of the development of a standard method of assaying nondestructive living samples from animal hair. Therefore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine clenbuterol-like beta-agonist residues in hair was developed and validated. The proposed LC-MS/MS method has high sensitivity and strong specificity with a relatively easy procedure for sample preparation. Thus, it can be used to detect beta-agonist residues in hair, providing a basis for regulating food safety.

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