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| Featured Webcasts: |
LIVE WEBCAST: Monday, January 24, 2011; 16:00 GMT
The CHROMacademy Essential Guide to Hydrophilic Interaction Chromatography (HILIC) and Related Techniques (Part 1)
The Essential Guide from LCGC’s CHROMacademy presents the first in a series of webcasts on Understanding Hydrophilic Interaction Chromatography and Related Techniques. Dr. Amos Heckendorf (The Nest Group) and Tony Taylor (Crawford Scientific Technical Director), present Part 1 in a series of Webcasts on these very popular and often misunderstood techniques, which allow retention of charged and highly polar analytes and offer an orthogonal selectivity to separations obtained in conventional reversed phase mode.
The session will consider what constitutes HILIC separation mechanisms and will compare this mode with Mixed Mode Chromatography to draw some important distinctions between these techniques and the way in which they are implemented. The main mechanisms of HILIC interaction will be considered with respect to eluotropic strength (high organic or high aqueous), pH effects on both the analyte and stationary phase surface and the influence of buffer strength and additives on the separation. In both HILIC and Mixed Mode Chromatography, the key to success is an understanding the nature of the interaction between the analyte and the mobile phase with the stationary phase surface, which may include hydrophilic interactions (adsorption), partitioning (into water rich layers at the stationary phase surface), electrostatic interaction with charged ligands, dipole and hydrogen bonding interactions. This webcast introduces the concepts and approaches to optimizing separation conditions for both HILIC and Mixed Mode Separations by considering, in detail, the nature of analyte / stationary phase / mobile phase interactions.
A must for anyone considering using HILIC or Mixed Mode mechanisms to overcome retention or detection issues with Reversed Phase separations or those wishing to optimize existing separations via orthogonal selectivity options.
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LIVE WEBCAST: Wednesday, January 26, 2011; 11:00 AM EST, 8:00 AM PST; 16:00 GMT
Sample Preparation Techniques for Ion Chromatography
Sample preparation is the key to successful ion chromatographic determinations when analyzing solid, airborne, or liquid samples. This webinar discusses the newest technologies to automate and simplify preparation of these types of samples. Sample combustion systems coupled to ion chromatographs have become a routine technique for halogen and sulfur determinations in solids. A solid is placed in an autosampler and all the steps required to obtain chromatographic data with quantitation are performed online. Practical applications for the analysis of halide anions in petrochemical and polymer samples will be presented. Automated air sampling with online ion chromatography is now straightforward. The combination of a fully automatic air separator and concentrator allows analysis of both particulates and volatile ionic constituents online. Modern ion chromatographs can run unattended for weeks so this methodology is well adapted to field applications for environmental surveillance. Finally, a review of automated pretreatment for liquid samples will be discussed. Applications in environmental, industrial, and power, as well as food will be presented highlighting new inline matrix elimination applications.
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LIVE WEBCAST: Tuesday, February 8, 2011; 11:00 AM EST
High Throughput/High Resolution Glycosylation Pattern Analysis of Monoclonal Antibodies by Capillary Electrophoresis
Structural characterization of the glycan moieties of therapeutic monoclonal antibodies is very important during both the clone selection and the production processes. Capillary electrophoresis (CE) in conjunction with laser induced fluorescent (LIF) detection is a sensitive and high resolution separation method that is already broadly utilized and validated in the analysis of complex carbohydrates in the biotechnology industry. By means of capillary electrophoresis, glycans released from subnanomolar amounts of antibodies can be quickly and accurately profiled. This presentation gives an overview of the state of the art of capillary electrophoresis based glycan analysis of
monoclonal antibodies, including sample preparation, glycan labeling and cleanup, oligosaccharide profiling, carbohydrate sequencing and glucose unit(GU) value based structural prediction. Rapid (<7 min) separation of 12 key IgG glycans will be shown along with 96 well plate operation for convenient overnight processing for such high throughput applications as clone selection.
The relative migration time reproducibility of the optimized separation method was RSD <0.09%. This facilitates peak assignment for individual components in any glycan pool, thus allowing high precision structural predictions using GU value databases. Exoglycosidase based sequence verification of IgG glycans will also be presented.
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| Featured Podcasts: |
Supercritical Fluid Chromatography in Theory and Practice
In this podcast series, we'll be discussing how users can benefit from supercritical fluid chromatography (SFC) in their laboratories. Amongst its many advantages, this technology can be utilized in both the analysis and purification of various chemical compounds, and is regarded as a green technology by many companies actively pursuing greater efficiency in their labs.
Optimizing Chromatographic Conditions for Protein Analysis with the ACQUITY UPLC H-Class Bio System
In this podcast series, we’ll be discussing how chromatographic analyses of biopharmaceuticals, particularly proteins, compare to the more familiar assays of small molecule drugs and the benefits that UPLC® Technology can offer.
Xevo G2 QTof: Answering the Quan/Qual Question, Comprehensively
As scientific challenges and goals continue to develop, today’s analytical laboratories are faced with the need for higher performing instruments. Waters’ innovations in quadropole/time of flight mass spectrometry technologies enable scientists to reach these goals.
The new Xevo G2 QTof mass spectrometer from Waters provides the highest quality and most comprehensive information, so you can make decisions better, faster and easier than before. This series of podcasts will address how the Xevo G2 QTof can deliver the results needed, with particular focus on non-targeted screening of food and environmental samples and also Proteomics Research.
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Xevo TQ-S: An introduction to the new, highly sensitive Mass Spectrometer from Waters
This series of podcasts will address the benefits of Xevo TQ-S and will show how this instrument can deliver the results needed, with particular focus on the analysis of pesticides in drinking water and food samples, and also the quantification of proteins and peptides.As scientific challenges and goals continue to develop, today’s analytical laboratories are faced with the need for higher performing instruments. Waters’ innovative tandem quadropole mass spectrometry technologies enable scientists to reach these goals.
The new Xevo TQ-S mass spectrometer from Waters provides the ultimate in versatility for a wide variety of applications, both quantitative and qualitative enabling you to make decisions better, faster and easier than before. |
| Featured Application Notes: |
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Which GPC Column First?
Most manufacturers recommend the installation of SEC columns in order of decreasing pore size when running columns in series. Scientists at Tosoh Bioscience tested the validity of this recommendation.
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