LCGC Europe Application Note Alert
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February 2015
Sample Prep
HPLC
 
Sample
Pressurized-Liquid Extraction for the Analysis of Diesel Range Organics (DRO) in Soil
The following application note focuses on the diesel range organics (DRO) constituents that can pose greater analytical difficulties than oil range organics (OROs) because of their increased volatility. The approach of pressurized-liquid extraction paired with gentle evaporation at low temperatures was chosen for optimal recoveries. Download the app note
Biotage® Extrahera™ vs. Manual Processing of Vitamin D Using ISOLUTE® SLE+
Automated sample preparation using the Biotage® Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Vitamin D analytes were extracted from pooled stripped plasma using an ISOLUTE® SLE+ procedure on 400 µL sample volume plates. Resulting extracts from both sample preparation methods were subsequently analyzed by LC–MS–MS. Download the app note

Sample
Profiling of Citrus Oils by 2D LC
This application note describes the analysis of citrus oils using the Agilent 1290 Infinity 2D LC solution with normal-phase LC in the first dimension and reversed-phase LC in the second dimension.
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Automated Extraction of Cortisone and Prednisolone from Human Plasma
The automated extraction of cortisone and prednisolone from human plasma using Novum SLE 96-well plates along with Tecan's Freedom EVO® 100 liquid handler yields quantitative recovery of both cortisone and prednisolone. The analytical method using a Kinetex core-shell Biphenyl HPLC/UHPLC column with MS-MS detection shows good selectivity for the two isomeric compounds.
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Accurate Pain Management Analysis
Pain management analyses using liquid chromatography can be difficult to optimize because of the limited selectivity of C18 and phenyl-hexyl phases. In contrast, the selectivity of Raptor™ Biphenyl superficially porous particle (SPP) LC columns provides complete resolution of isobaric pain medications with a total cycle time of 5 min.
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LC-MS-MS Method for Psychedelic Phenethylamine “2C” Series on a SELECTRA®  DA HPLC Column
A new group of "2C" compounds – belonging to the psychoactive phenethylamine class of designer drug – has recently emerged on the market known as the N-methoxybenzyl (‘NBOMe) derivatives of substituted phenethylamine. This application note outlines an LC-MS-MS method using a unique polyaromatic phase to separate 16 "2C" compounds in under 10 min. Download the app note

Sample
Absolute Molar Mass in UHPLC via the µDAWN and UT-rEX
Ultrahigh-performance liquid chromatography (UHPLC) enables fast, efficient separation of biomolecular samples. Compared to standard SEC, UHPLC-SEC provides improved resolution, higher throughput, less solvent, and smaller sample consumption for analysis of precious biological samples. Download the app note
Investigating the Effect of Molecular Size, Weight, and Structure on Product Performance
A selection of different PLA and PLGA polymer samples were analyzed on Malvern's OMNISEC GPC/SEC system, which combines multiple advanced detectors to provide information about structure and solution properties. The data shown in this application note allows clear differentiation between different compositions of PLGA by the different properties the samples exhibit in solution. These differences will affect product performance such as degradation and drug delivery rates.
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