In our August issue:
COLUMNS
COLUMN WATCH
Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry
Craig R. Aurand, Hugh Cramer, Jacinth McKenzie, and David S. Bell
If your favorite C18 column doesn’t provide good results, what is your next step? Here is some advice. Read more >> |
LC TROUBLESHOOTING
Estimating Resolution for Marginally Separated Peaks
John W. Dolan
How can resolution be determined when peak width cannot be measured? Read more >> |
MS – The Practical Art
Quantifying Proteins by Mass Spectrometry
Mark W. Duncan, Alfred L. Yergey, and P. Jane Gale
Confused about the differences between top-down, bottom-up, and shotgun strategies to protein quantitation? And what about labeled and unlabeled approaches? This article breaks it all down. Read more >> |
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At-a-Glance Views to Help Maximize Laboratory Resources
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Biotechnology Today
The Role of Elution Gradient Shape in the Separation of Protein Therapeutics
Varsha S. Joshi, Vijesh Kumar, and Anurag S. RathoreLinear gradients are easier to manage, but nonlinear gradients offer distinct advantages, including improved sample resolution and increased detection sensitivity. All you need is a systematic approach to optimizing them. Here’s how. Read more >> |
The Essentials
Critical Evaluation of HPLC Methods
An investigation into some “typical” HPLC method specifications highlights ways of critically evaluating the major and minor parameters of a method. Read more >> |
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PEER-REVIEWED ARTICLES
Comparison of Enantiomeric Separations and Screening Protocols for Chiral Primary Amines by SFC and HPLC
Ross M. Woods, Zachary S. Breitbach, and Daniel W. Armstrong
Immobilized chiral selectors based on derivatized cyclofructans, such as CF6, have been shown to provide excellent selectivity for a variety of racemic compounds. However, no comprehensive study on the use of CF6 had been performed under SFC conditions — until now. Read more >> |
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