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Novel Approaches for Small Molecule Identification
Gabriela Zurek, and Aiko Barsch, Bruker Daltonics, Inc.
In plant metabolomics, confirmation of the structure of known compounds and structural elucidation of unknown compounds represents a bottleneck in the analysis of secondary metabolites. In this application note, we present novel tools that facilitate the identification process of natural products when mass spectral libraries are not available and sample amounts are limited. This information can subsequently be used to build well-characterized MS–MS libraries, enabling quick dereplication of known compounds. Read More |
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Accurate Compound Measurement in Metabolomics: Isotope Ratio Outlier Analysis (IROA) Protocol
Kevin J. McHale, Mark Szewc, Gregory S. Stupp, Chaevien Clendinen, Ramadan Ajredini, Arthur S. Edison, and Chris Beecher, Thermo Fisher Scientific
The combination of IROA and HR/AM LC–MS–MS enables metabolites to be distinguished in an untargeted manner. Download this application note and learn more about metabolite quantitation using IROA and the Thermo ScientificTM Q ExactiveTM mass spectrometer. Read More |
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Polycaprolactam for Controlled Drug Delivery — Analysis on Agilent PLgel MIXED-C
Stephen Luke, Agilent Technologies
The polymer polycaprolcatam allows for the steady release of a drug within the body as it degrades. This application note describes the measurement of the molecular weight distributions of this biodegradable polymer by GPC, a critical part of research into controlled drug release. Read More |
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Speed Up the Delivery and Improve the Quality of your Bioformulation Stability Data
Malvern Instruments
Analyzing oligomeric composition and determining protein molecular weight and size is made possible by combining the power of a highly sensitive light scattering instrument with your size-exclusion chromatography (SEC) system. This application note describes how aggregated material in a protein sample can be detected and accurately quantified. For formulation studies, batch dynamic light scattering measurements down to 2 uL are also possible using the same instrument. Read More |
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Solid-Phase Extraction of Base, Neutral and Acid Semi Volatiles in Industrial Waste Water
Phillip Bassignani, FMS,Inc.
Traditionally involving 6 LLE shakes at both pH 2 and 12 (plus heavy emulsions and low recoveries), traditional base, neutral, and acid for semi volatiles extractions are time-consuming and often lead to poor results. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semi volatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labour. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semi-volatile extraction process. Read More |
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Extraction of Antiepileptic Drugs from Serum and Urine Using ISOLUTE® SLE+ Prior to LC–MS–MS Analysis
Victor Vandell, Biotage AB, Sweden
This application note describes the extraction of neutral and zwitterionic antiepileptic (AED) drugs from serum and urine using ISOLUTE SLE+ in a 96-well plate format. The method demonstrates a quick, simple, and high-throughput method with high levels of recovery achieved. Read More |
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A Universal Tool for Method Transfer from HPLC to UHPLC
Holger Franz and Susanne Fabel, Thermo Fisher Scientific
This Technical Note presents the theoretical background and introductions equations for an application's transfer to UHPLC. Read More |
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Ephedra Alkaloids Analysis Using SCX SPE and LC-MS/MS on a PFPP HPLC Column
Brian Kinsella, UCT, Inc.
This method describes the extraction, cleanup, and quantification of 5 ephedra alkaloids and synephrine in functional foods and natural products. A high capacity, strong cation-exchange SPE cartridge was used for the isolation of the phenethylamines from dietary supplements. HPLC separation, including separation of the enantiomers, was carried out using a UCT Selectra® PFPP column prior to detection by LC-MS/MS. Read More |
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Characterization of PLGA Using SEC–MAL–VIS
Wyatt Technology Corporation
The characterization of PLGA, by means of conventional size-exclusion chromatography (SEC), is problematic because of the lack of suitable calibration standards that renders conventional column calibration an inadequate analytical technique. SEC–MALS–VIS is an excellent method for the characterization of PLGA polyesters as it has the ability to determine not only the molar mass distribution, but also to reveal subtle differences in PLGA’s molecular structure. Read More |
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Direct Analysis of Multicomponent Vaccine Adjuvants by HPLC with Charged Aerosol Detections
Dave Thomas and Ian Acworth, Thermo Fisher Scientific
This application note describes a fast and sensitive HPLC method suitable for the strength and purity of immunological adjuvant formulations. Read More |
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