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Product Authentication and Adulteration Determination Using a Novel Spectro-Electro Array Platform
Paul A. Ullucci, Marc Plante, Ian N. Acworth, Christopher Crafts, and Bruce Bailey, Thermo Fisher Scientific
A gradient high-performance liquid chromatography (HPLC) spectro-electro array platform combines the universality of diode array detection with the selectivity and sensitivity of coulometric electrode array electrochemical (EC) detection. This technique can be used to simultaneously measure hundreds of known and unknown secondary metabolites in a single sample.
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Determination of Dicyandiamide in Milk Powder
Chen Jing, Dai Zhenyu, Xu Qun, Liang Lina, and Jeffrey Rohrer, Thermo Fisher Scientific
Dicyandiamide is applied to dairy pastures to reduce the rate at which soil microbes convert ammonia from animal waste into nitrates and nitrous oxide, thus slowing nitrate leaching. Its overuse can result in the compound appearing in dairy products. The ion-exchange HPLC method for dicyandiamide analysis described in this application note simplifies the sample preparation process and eliminates the cartridge cleanup procedure, thus reducing analysis time and cost.
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Direct Analysis of Multicomponent Vaccine Adjuvants by HPLC with Charged Aerosol Detection
Dave Thomas and Ian Acworth, Thermo Fisher Scientific
This application discusses how the lack of a detectable chromophore in several adjuvant components and degradation products can be overcome by using HPLC with charged aerosol detection, a detection method that can measure any nonvolatile compound. Charged aerosol detection has greater sensitivity and precision than evaporative light scattering detection and refractive index detection, is fully gradient compatible, and is simpler to operate than a mass spectrometer.
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Simultaneous Determination of Amlodipine and Its Counterion Besylate by HPLC
Brian De Borba and Jeffrey Rohrer, Thermo Fisher Scientific
This study focuses on an HPLC method to simultaneously separate the active pharmaceutical ingredient and counterion of the drug substance amlodipine besylate, a calcium channel blocker used for the treatment of hypertension and angina. A trimode column was used that provides spatial separation of the anion-exchange, cation-exchange, and reversed-phase modes, which allows each mode to be independently controlled.
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The Vanquish Platform: Major Improvement in Throughput and Resolution of Xanthones in Mangosteen Pericarp
Qi Zhang, Bruce Bailey, Marc Plante, and Ian Acworth, Thermo Fisher Scientific
Recently mangosteen, a tropical fruit indigenous to Southeast Asia, has been proposed as a
homeopathic therapy in the treatment of Parkinson's disease. The UHPLC approach used in this study of xanthones in the pericarp of mangosteen provides enhanced throughput with better resolution and peak capacity, with an analysis time of 11 min.
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Fast Nevirapine Impurity Profiling Using UHPLC-DAD
Holger Franz and Susanne Fabel, Thermo Fisher Scientific
Nevirapine is a non-nucleoside reverse transcriptase inhibitor currently marketed for the treatment of HIV-1 infected adults. This application note discusses a state-of-the-art ballistic gradient UHPLC–UV method for the analysis of nevirapine and its impurities.
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Separation of Papain Digests of Oxidized Monoclonal Antibodies
Dai Zhenyu, Xu Qun, Lina Liang, and Jeffrey Rohrer, Thermo Fisher Scientific
Many monoclonal antibodies have been developed as human therapeutics, with several more under development. This application note describes the use of hydrophobic interaction chromatography (HIC), a method that is partially or fully orthogonal to cation-exchange and size-exclusion chromatography, for separating and purifying antibody fragments generated by papain digestion.
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Separation of Intact Monoclonal Antibody Sialylation Isoforms by pH Gradient Ion-Exchange Chromatography
Dai Zhenyu, Xu Qun, Lina Liang, and Jeffrey Rohrer, Thermo Fisher Scientific
This application describes a pH-gradient-based strong cation exchange chromatography approach that provides excellent resolution for monoclonal antibody charge variants. Compared to routine isoelectric focusing methods, this method is more convenient and straightforward for protein quality control.
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High Resolution Peptide Mapping for Biopharmaceutical Analysis
Mauro De Pra, Thermo Fisher Scientific
Protein digests are often very complex samples, containing tens or even hundreds of peptides. The method described in this study uses gradient reversed-phase HPLC with a C18 column at elevated temperature to achieve excellent peak capacity with relatively short run times.
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Rapid and Sensitive Determination of Rhodamine B in Cosmetics
Huang Xiongfeng, Xu Qun, and Jeffrey Rohrer, Thermo Fisher Scientific
Rhodamine B is a brightly colored synthetic pigment that has been extensively used as a dye in many industries. Long-term contact with the compound may cause cancer and birth defects. This work describes an efficient HPLC method that uses fluorescence detection to achieve a rapid and sensitive determination of rhodamine B in cosmetics.
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