LCGC Europe Single Sponsored Application Note Alert Having trouble viewing this email? Click here

October 2014 Food Safety Water Analysis Forensics and Drug Testing Drug Development Biochemical Peptides and Proteins Improved Determination of Antioxidants in Edible Oils Using Solid Core LC Columns Mark Tracy, Thermo Fisher Scientific Phenolic antioxidants are commonly used as preservatives in foods containing edible oils and fats to prevent development of rancidity. AOAC Method 983.15 is used to assay levels of these compounds in finished food products. The method uses a C18 column but can suffer from matrix interference effects.  Download the app note Rapid Screening Method for Veterinary Antibiotics Using an Advanced Solid Core UHPLC Column and UHPLC System with MS-MS Detection Gordon Wilson and Derek Hillbeck, Thermo Fisher Scientific This application shows the UHPLC analysis of 36 antibiotics at high flow rates. The need for chromatographic separation of isobaric compounds prior to MS detection is also highlighted. Download the app note Rapid Separation of 18 Herbicides Using Advanced UHPLC and Sub-2 µm Solid Core Column Technologies Kristina Kirkham, Luisa Pereira, and Derek Hillbeck, Thermo Fisher Scientific This application shows the UHPLC separation of 18 herbicides. The higher flow rates used in this approach enable development of rapid, high-performance analytical methods. Download the app note Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged Aerosol Detection Mark Tracy, Xiaodong Liu, and Ian Acworth, Thermo Fisher Scientific Polyacrylic acid (PAA) is widely used as an antiscaling additive for boiler water in steam generators. This work demonstrates the application of size-exclusion chromatography and charged aerosol detection to the measurement of PAA in boiler water. No sample extraction or pre-concentration is needed to attain detection limits below 10 µg/L.  Download the app note Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS-MS Detection Derek Hillbeck, Thermo Fisher Scientific This application shows the advantages of using UHPLC for the analysis of 47 illicit drugs. The technique's high flow rates enable development of rapid analytical methods while maintaining performance. Download the app note High-Resolution Separation of Oligonucleotides on a Pellicular Anion-Exchange Column J.R. Thayer, Thermo Fisher Scientific Multiple oligonucleotide-based therapeutic models have been developed to date. Mechanisms to resolve oligonucleotides from their modified forms, synthetic failure fragments, and their metabolic products can be technically challenging to develop. This article discusses the importance of selectivity and resolution for the separation of therapeutic and diagnostic oligonucleotides. Download the app note High-Resolution Separation of Intact Monoclonal Antibody Isoforms Dai Zhenyu, Xu Qun, and Jeffrey Rohrer, Thermo Fisher Scientific Glycosylated proteins constitute a large portion of major approved therapeutic protein drugs. This article describes a fast, reproducible, high-resolution, ion-exchange chromatography approach for the separation of monoclonal antibody sialylation isoforms using a pH gradient buffer. Download the app note Separation of Biochemical Buffering Agents Using Multi-Mode Liquid Chromatography with Charged Aerosol Detection Mark Tracy and Xiaodong Liu, Thermo Fisher Scientific Good's buffers are a series of zwitterionic buffering agents popularly used in biochemical experiments and HPLC mobile phases. We present analytical conditions to separate 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions and charged aerosol detection.  Download the app note Rapid Separation and Mass Spectrometry Detection of Intact Protein Samples Using Monolithic Capillary Columns Shane Bechler and Kelly Flook, Thermo Fisher Scientific This application note demonstrates the rapid separation of peptides and proteins using a reversed-phase monolithic column with both UV and MS detection. These simple, straightforward methods can be used to optimize chromatographic separations for analysis time, resolution, and MS detection especially for top-down proteomics. Download the app note   Go to Application Note Library Subscribe to the Application Note Alert LCE Print Edition LCE Digital Edition