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Ultra-Sensitive Detection of Pharmaceutical and Personal Care Products in Water with an Integrated On-Line Extraction (OLE)-UHPLC–MS–MS System
Zicheng Yang, Helen (Qingyu) Sun, and Kefei Wang
An advanced ultrahigh-pressure liquid chromatography (UHPLC) method was developed for the quantification of trace pharmaceutical and personal care products (PPCPs) in clean water. The Bruker Advance™ UHPLC system, with integrated on-line extraction (OLE) coupled to the EVOQ LC–MS-MS detected 1–2 ppt levels of PPCPs in water with a linear response up to 200 or 500 ppt. Excellent system robustness was obtained throughout the extended method development and sample analysis period. Read More |
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Quantitative LC–MS–MS Analysis of Tetracycline Antibiotics in Honey Using Advance UHPLC–EVOQ™ Elite LC–MS–MS system
Gouri Satpathy and J.S Chadha
A simple, robust, and effective LC–MS–MS method was developed for simultaneous detection and quantification of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), and doxycycline (DC) in food grade honey. Analytes were extracted in EDTA-McIlvaine buffer from honey by shaking and ultra-sonication followed by extraction using C18 cartridge and further analyzed by LC–MS–MS using multiple reaction monitoring mode. Responses of all target analytes were found linear between 1.0 to 200 ppb with correlation coefficients (r2) >0.998. The method was validated according to European Commission Decision 2002/657/EC. The limit of quantification (LOQ) achieved was in accordance with MRL specified for tetracyclines. Overall recoveries for all tetracyclines were found to exceed 79% with repeatability of <10%, thus meeting analytical method requirements. Read More |
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Evaluation of Matrix Effects for Dilute–and–Shoot LC–MS–MS Analysis of Carbendazim in Orange Juice and Wine
Helen (Qingyu) Sun, Zicheng Yang, and Kefei Wang
A quantitative multiple reaction monitoring (MRM) method for carbendazim analysis in orange juice and wine matrices was developed for the Bruker EVOQ Elite LC–MS–MS system with a calibration range from 0.005 ppb up to 50 ppb. Matrix effects were evaluated by comparing matrix effect percent against dilution factors. Results indicate that orange juice and wine matrices exhibit different matrix effects and provide the scientific reference to determine appropriate dilution factors. Good instrument sensitivity and robustness were achieved during method development. Read More |
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Use of GC–MS for Optimal Quantification of THC in Blood Samples
D. Debruyne, M. Loilier, A. Hurbain, and A. Collgros
A GC–MS method has been developed for the analysis THC and THC-COOH in blood samples on the SCION SQ single quadrupole gas chromatography mass spectrometer. The method demonstrated the reliable and robust quantitation of 0.5 ng/mL THC and 2.5 ng/ mL THC-COOH in blood extracts, while excellent instrument robustness and linearity is demonstrated throughout a calibration range of 0.5 to 10 ng/mL for 9-THC and 2.5 to 50 ng/mL for THC-COOH. Read More |
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Use of GC–NCI–MS–MS for Quantification of THC from Hair Samples
A. Collgros and A. Hurbain
A GC–NCI–MS-MS method has been developed for the analysis of THC-COOH in hair samples on the SCION TQ™ triple quadrupole gas chromatography mass spectrometer. The method is sensitive to detect 0.05 pg/mg of THC-COOH, the limit of detection set by regulations in the Unites States and many European countries, while excellent instrument robustness and linearity are demonstrated throughout. Read More |
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Rapid and Simple Approaches to Multi-Residue Pesticide Analysis in Fruits and Vegetables on both GC–MS–MS and LC–MS–MS
Helen (Qingyu) Sun, Zicheng Yang, and Kefei Wang
This study demonstrates a multi–residue pesticide analysis method for complex food matrices prepared by a modified QuEChERS protocol using a Bruker SCION GC–MS–MS and EVOQ LC–MS–MS system. The sample preparation method is well suited for direct injection on both GC–MS–MS and LC–MS–MS systems. The compound based scanning (CBS) feature enables fast multiple reaction monitoring (MRM) method development on both the EVOQ and SCION. Excellent linearity was achieved on both instruments and good sensitivity of low ppb (1 ppb) for GC–MS–MS analysis and sub-ppb (0.1 ppb) for LC–MS–MS analysis is demonstrated. Read More |
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Quantitative LC–MS–MS Analysis of Sulfonamides in Honey Using Advance UHPLC-EVOQ™ Elite LC–MS–MS System
Gouri Satpathy
A simple, robust, and effective LC–MS–MS method operated in multiple reaction monitoring (MRM) mode was developed for simultaneous estimation of “sulfonamide drugs” in food grade honey with easy and minimal sample preparation. In this experiment, two MRM transitions were tracked for each targeted analyte, one for quantification and the other for qualification. The limit of quantification achieved for all sulfonamides was 0.1 ppb with consistent ion ratio of quantifier to qualifier ions. The method was found to be linear and repeatable with regression (R)2 of 0.996 and relative standard deviation of <12% for all nine sulfonamides under study. The use of the EVOQTM Elite Triple Quad LC–MS–MS system offers high sensitivity, specificity, and flexibility of the method and allows high sample throughput for screening and quantification of sulfonamides in honey. Read More |
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LC–MS–MS Analysis of Immunosuppressant Drugs in Human Plasma Using EVOQ™ Elite LC–MS–MS System
Shashin Shah
An LC–MS–MS method has been developed for analysis of four immunosuppressant drugs, namely, tacrolimus, sirolimus, everolimus, and cyclosporine A, in human plasma. The method is sensitive to detect 0.5 ng/mL level of tacrolimus, sirolimus, everolimus, and 10 ng/mL of cyclosporine A. The linear calibration range of 0.5 to 50 ng/mL was achieved for tacrolimus, sirolimus and everolimus, and 10 to 1000 ng/ml for cyclosporine A. Read More |
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